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. 2024 Jun 11;16(1):2363012. doi: 10.1080/19490976.2024.2363012

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© 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 3. — Bacterial enrichment in CRC adjacent normal is associated with CIMP.

(a) Scatterplot showing the enrichment of 115 paired tumor samples (y-axis) and adjacent normal samples (x-axis) by qPCR in B. fragilis, F. nucleatum, and K. pneumoniae. Linear regression lines (black) are built on each correlation. Pearson correlation coefficients and p-values are indicated in each plot. (b) Comparison of the delta Ct values between 115 paired adjacent normal and the tumor samples by bacterial qPCR. Blue and cream lines connect each paired adjacent normal and tumor samples. Blue dots represent bacterial Superhigh cases determined by high bacterial enrichment in tumor samples across the three bacterial species. (c) Comparison of the Superhigh and the Non-Superhigh cases determined by tumor samples in adjacent normal samples. Significance was calculated by Wilcoxon test.