Table 2. Formulations and experimental conditions.
| Category 1: Enzymatic alkanolamine surfactant-based formulations | |||||
| Formulation | Composition# (v/v), condition | TESSA reactions (positive/total)* | |||
| F1, F2 | 20D / 10U,10V | 4/4* | |||
| F3-F5 | 20D / 11T-11V | 4/4* | |||
| F6-F9 | 20D / 40B-40E | 4/4* | |||
| F10, F11 | 20H / 10U, 10V | 4/4* | |||
| F12 | 20H / 11T | 8/16 | |||
| F13 | 20H / 11U | 7/12 | |||
| F14 | 20H / 11V | 2/8 | |||
| F15-17 | 20H / 40B-40D | 4/4* | |||
| F18 | 20H / 40E | 4/12 | |||
| F26 | 20H / 12A | 7/20 | |||
| F27 | 20H / 12B | 5/8 | |||
| F28-F30 | 20H / 12C-12E | 3/4* | |||
| F32 | 20K / 12F | 6/8 | |||
| F38-F50 | 20K / 12K-12W | 4/4* | |||
| F51-F56 | 20K / 13A-13F | 4/4* | |||
| F57 | 20K / 13G | 8/8 | |||
| F58 | 20K / 13G, 20 min, 55°C | 8/8 | |||
| F59 | 0.4% 20K / 0.6% 13G | 7/8 | |||
| F60 | 0.4% 20K / 0.6% 13G; 20 min, 55°C | 4/8 | |||
| F61 | 0.4% 20K / 0.6% 13G; 20 min, 65°C | 6/8 | |||
| *: All formulations in this row yielded identical results. | |||||
| 20D: Alkanolamine 30–50% (complexing agents 1–5%, amines > 30%, phosphonates <1%) | |||||
| 20H: Alkanolamine 30–50% (complexing agents 5–10%, amines >30%, phosphonates <1%) | |||||
| 20K: Alkanolamine 5–15% (complexing agents 5–15%, amines 5–15%, phosphates 15–30%, phosphonates <1%) | |||||
| 10U-V: enzymatic product with different concentrations of Ca- and Mg-salts, enzymatic components 1–5% | |||||
| non-ionic surfactant 1–5% | |||||
| 11T-V: various enzymatic products, non-ionic surfactants, different concentrations of fatty alcohol alcoxylates 5–10%, enzymatic components <5% and alkylglucoside, glycol 1–5% | |||||
| 12A-W: 11T, various concentrations of ingredients | |||||
| 13A-G: various enzymatic products, non-ionic surfactant | |||||
| 40B-E: 11T-U, lower enzyme concentrations, higher glycol concentration, non-ionic surfactants | |||||
| # Formulations F1-F58 are composed of 0.3% (v/v) 20D-20K and 0.2% (v/v) 10U, 10V, 11T-11V, 40B-40E, 12A-13W or 13A-13G | |||||
| Category 2: Enzymatic surfactant phosphate/silicate-based formulations | |||||
| Formulation | Composition#, condition | TESSA reactions (positive/total) | |||
| F19 | deconex® TWIN PH10 / 11T | 5/16 | |||
| F20 | deconex® TWIN PH10 / 11V | 2/4 | |||
| F21 | deconex® TWIN PH10 / 12A | 2/8 | |||
| F22 | deconex® TWIN PH10 / 12B | 2/8 | |||
| F23 | deconex® TWIN PH10 / 12C | 4/8 | |||
| F24 | deconex® TWIN PH10 / 12D | 4/8 | |||
| F25 | deconex® TWIN PH10 / 12E | 3/4 | |||
| F31 | deconex® TWIN PH10 / 12F | 4/8 | |||
| 11T-11V: various enzymatic products, non-ionic surfactants, different concentrations of fatty alcohol alcoxylates 5–10%, enzymatic components <5% and alkylglucoside, glycol 1–5% | |||||
| 12A-F: 11T, various concentrations of ingredients | |||||
| #Formulations are composed of 0.3% (v/v) Dec TWIN PH10: Phosphate/Silicate and 0.2% 11T, 11V or 12A-12F | |||||
| Category 3: Hydroxide-based products and solutions | |||||
| Formulation | Composition (v/v), condition | TESSA reactions (positive/total) | |||
| F67 | 1% deconex® OP 152, 40 min | 8/16 | |||
| F68 | 1% deconex® HT 1501,40 min | 0/16 | |||
| F69 | 1% deconex® MT 14, 40 min | 0/16 | |||
| F74 | 0.5% deconex® MT 14 | 3/8 | |||
| F72/73 | 1% deconex® MT 14 | 4/24 | |||
| F75 | 2% deconex® MT 14 | 4/8 | |||
| F76 | 3% deconex® MT 14 | 3/8 | |||
| Category 4: Hypochlorite-based formulations | |||||
| Formulation | Composition (v/v)#, condition | TESSA reactions (positive/total) | |||
| F65 | 0.5% 70B, 0.5% enzyme and surfactant, 40 min | 1/8 | |||
| F66 | 1% 70B, 40 min | 0/16 | |||
| F71 | 1% 70B | 9/24 | |||
| F71.2§ | 1% 70B, freshly prepared | 0/16 | |||
| F77 | 0.3% 70B | 4/20 | |||
| F77.2 | 0.3% 70B, 5 min, freshly prepared | 2/8 | |||
| F78 | 0.5% 70B | 4/20 | |||
| F78.2 | 0.5% 70B, 5 min, freshly prepared | 0/8 | |||
| F81 | 0.75% 70B | 3/8 | |||
| 70B: Tetrapotassium pyrophosphate (15–30%), dipotassium trioxosilicate (5–15%), sodium hypochlorite solution (active chlorine (< 5%)) | |||||
| § Graphs are presented in Fig 2B and S2B Fig. | |||||
Post-hoc classification of the formulations and experimental conditions. The number of positive replicates from up to 5 independent TESSA experiments with 4 replicates each are shown. All formulations were applied under machine reprocessing conditions (55°C, 10 min), if not otherwise indicated.