Figure 7.

Enhanced liver regeneration induced by SG is not a consequence of NAFLD remission. (A) Experimental flowchart of SG/Sham combined hepatectomy. (B) BrdU staining results showed that SG can directly enhance liver regeneration. A comparison of SG versus Sham results indicates that the enhanced liver regeneration by SG is not caused by the alleviation of fatty liver caused by SG itself. (C) The H&E results showed that the liver status of both groups was basically the same, and there was no histological damage caused by the surgery. (D) Liver function blood biochemistry results indicate that the physiological condition of the livers in both mouse groups is in a similar healthy state. (E) The results of PCNA immunohistochemical staining also confirmed the presence of enhanced liver regeneration. (Data are shown as mean±SE. ns=P>0.05, *=P<0.05, and ****=P<0.0001. For panel D, n=5 Sham, 5 SG. For panels B and E, seven visual fields were collected to calculate BrdU and PCNA positive rates, respectively). NAFLD, non-alcoholic fatty liver disease; SG, sleeve gastrectomy.