FIG. 2.

Resonance energy transfer from NBD-labeled X-47 to rhodamine-labeled A/Shangdong HAs. Both HAs were reconstituted together, at a 1:1 ratio (thin line), or the HAs were reconstituted separately and the resulting membranes were mixed 1:1 after reconstitution (bold line). Data are for 10 nmol of virosomes in 2 ml of buffer at pH 7.4, 0°C. HAs were labeled and reconstituted as described in Materials and Methods.