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. 2024 Jun 13;14:13655. doi: 10.1038/s41598-024-64368-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transmission Electron Microscopy (TEM) analysis of mitochondrial morphology. (A) Representative TEM images of ultra-thin sections of cardiac mitochondria isolated from Wt, Taz^KD, Taz^KD + saline and Taz^KD + SS-31 mice (14,000X, scale bar = 1 μm). Magnifications (56,000X, scale bar = 0.2 μm) are also shown. (B) The violin plots represent the median of values and quartiles of: (a) the number of vacuolated mitochondria expressed as percentage of total number of mitochondria, (b) the vacuoles area expressed as percentage of total mitochondrial area, and (c) the cristae density expressed as percentage of the mitochondrial area occupied by cristae, in the four groups of animals. p-value < 0.05 was considered as statistically significant (one-way ANOVA or Kruskal–Walli’s test, **p < 0.01; ***p < 0.001; ****p < 0.0001).