Figure 2.

Immunoblotting analysis of proteins involved in mitochondrial fission. Western blot analysis of FIS1, DRP1, p-DRP1(s637), p-DRP1(s616) and porin protein expression in the heart of Wt and Taz^KD mice (a, e), and Taz^KD + saline and Taz^KD + SS-31 mice (c, m). Isolated cardiac mitochondria (for a, c) or total heart homogenates (for e, m) were separated by SDS-PAGE, transferred onto PVDF membrane, and then subjected to immunoblotting analysis with specific antibodies, as indicated. The histograms show quantification of the protein expression levels: FIS1 (b, d), DRP1 (f, n), p-DRP1(s637)/DRP1 ratio (g, o), p-DRP1(s616)/DRP1 ratio (h, p), p-DRP1(s616)/p-DRP1(s637) ratio (i, q) , porin (l, r), in the heart from the four groups of animals, as indicated. Data are presented as means + SEM; n = 3–4 mice per group. Taz^KD and Taz^KD + SS-31 data are represented as the percentage change from Wt and Taz^KD + saline, respectively, set on 100%. p-value less than 0.05 was considered as statistically significant (Student’s t test, *p < 0.05, **p < 0.01). Original blots are presented in Supplementary Figure S1.