Figure 3.

Immunoblotting analysis of proteins involved in mitochondrial fusion. Western blot analysis of L-Opa1, S-Opa1, YME1L, OMA1 protein expression in the heart of Wt and Taz^KD (a, d), and Taz^KD + saline and Taz^KD + SS-31 mice (g, l). The histograms show quantification of the protein expression levels: L-Opa1 (b, h), S-Opa 1 (c, i), YME1L (e, m) and OMA1 (f, n), in cardiac mitochondria from the four groups of animals, as indicated. Data are presented as means + SEM; n = 3–4 mice per group. Taz^KD and Taz^KD + SS-31 data are represented as the percentage change from Wt and Taz^KD + SS-saline, respectively, set on 100%. p-value less than 0.05 was considered as statistically significant (Student’s t test, **p < 0.01; ***p < 0.001). Original blots are presented in Supplementary Figure S2.