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. 2024 Jun 13;15:5059. doi: 10.1038/s41467-024-49504-6

Fig. 5. BnaC03.MPK3 and BnaC03.MPK6 act genetically downstream of BnaA07.MKK9 to regulate Sclerotinia stem rot (SSR) resistance.

Fig. 5

a–f Relative expression (a, d), lesion areas (b, e), and disease symptoms (c, f) of BnaC03.MPK3/BnaC03.MPK6 overexpression (OE) transgenic plants and J9712. g–j Relative expression (g, h), lesion areas (i), and disease symptoms (j) of J9712, Bnamkk9, BnaC03.MPK3 OE/Bnamkk9, and BnaC03.MPK6 OE/Bnamkk9. For qRT-PCR in (a, d, g, h), BnaUBC9 was the reference gene. k–m Relative expression (k), lesion areas (l), and disease symptoms (m) of Col-0, Csv::BnaA07.MKK9DD, AtMPK3SR and Csv::BnaA07.MKK9DD/AtMPK3SR Arabidopsis plants. qRT-PCR was performed at 12 h after methoxyfenozide (MOF) and NA-PP1 treatment. AtEF-1a served as the reference gene. The S. sclerotiorum inoculated leaves were photographed and quantified at 30 h post-inoculation (hpi). Scale bar: 1 cm. In (b, c, e, f, i, j), the S. sclerotiorum inoculated leaves were photographed and quantified at 48 hpi. Scale bar: 1 cm. In (a, d, g, h, k), values are means ± SD of three biological replicates. In (b, e, i, l), each bar represents the mean ± SD, and n represents the number of plants. In (a, b, d, e, g, h, k), statistical significance was determined by a two-tailed Student’s t-test. In (a, b, d, e,), significance compared with J9712. In (g, h), significance compared with Bnamkk9.In (i, l), different lowercase letters indicate significant differences (P < 0.05), determined with one-way analysis of variance (ANOVA) and Tukey’s honestly significant difference (HSD) post hoc test. Source data are provided as a Source Data file.