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. 2024 Jun 13;15:5025. doi: 10.1038/s41467-024-49117-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Pooled sera from the indicated number of humans for each decade of birth were tested for antibodies to H1N1pdm09 (red bars), γ-swH1N1 (purple bars), and α-swH1N2 (green bars) by HAI (A) and neutralization (B) assay. Data are presented as mean values +/− standard deviation (SD) from two biological replicates. Solid line in A indicates an HAI titer of 40, which corresponds to a 50% reduction in the risk of influenza virus infection. C Sera from individuals (N = 6) vaccinated in October 2021 (14 to 21 days post-vaccination) were assessed for cross-reactive neutralizing antibodies. Each dot represents an individual sample and is an average of 2 technical replicates. The colored lines represent the mean values between all the individual biological samples. For A–C, dashed lines indicate the limit of detection for each assay.