Fig. 1. IAR T-PIT TCR sequence matching.

Panels A–G utilized TCRs from Cohort1; panels H-K, TCRs from combined Cohorts 1 + 2. A) Perfectly matched IAR TRA and TRB junctions from Cohort 1 (Table 2) in PIT TCRs (n = 9,757 unique TRA and TRB junctions, Table 2). B Perfectly matched PIT TRA and TRB junctions in IAR TCRs (n = 4,310 unique TRA and TRB junctions). C Perfectly matched PIT T cell TCR junctions from CD4+ and CD8+ cells in IAR TCRs. D Perfectly PIT-matched versus non-matched TRA junctions from Cohort 1 IAR T cell TCRs (n = 55 matched and 2119 non-matched) versus TRA junctions from HC²⁰ (185 matched and 24,968 non-matched) and COVID-19 patients²⁰ (1341 matched and 197,412 non-matched). E As in D but using TRB junctions (n = 7 matched and 2129 unmatched). F PIT junction matching in IAR TRA junctions versus junctions from HC donors²⁰. Dot sizes, Levenshtein index values. Diagonal line, equivalency line. Blue line, best fit line from linear modeling. The pAdj for a slope different from 1 was calculated using linear modeling and ANOVA. Gray shading, 95% confidence intervals. G As in F but using TRB junctions. H PIT matching by different TRA chain segments in combined Cohorts 1 and 2 (n = 3264 unique TRA junctions). Lv0 and Lv1, Levenshtein index values of 0 and 1 (n = 74 and 2025 unique TRA junctions, respectively). I PIT matching by TRA junction segments in combined Cohorts 1 and 2 according to HLA-DRB1 alleles in PIT donors. There were: n = 484, 153, 199, and 95 junctions from donors having 03:01, 04:01, 07:01, and other HLA-DRB1 alleles, respectively. J Junction sharing by TCRs with PIT-matched TRA junctions. One TCR in each group recognized¹³ the indicated autoantigen epitope. K Total TRA chains (Table 2) in expanded (n = 1000) and non-expanded (n = 2907) cells from combined Cohorts 1 and 2 were tested for differences in PIT-matched and non-PIT-matched junctions from HC, Aab + , newT1D, and T1D donors using two-sided Fisher’s exact tests.