Figure 3.
The ΔccpC strain exhibited insignificant intracellular replication in macrophages compared to the wildtype F2365 strain. (A) The intracellular replication of the ΔccpC is reduced insignificantly by 0.4 log10 compared to F2365 based on CFU recovered from infected macrophages after 5 h (p > 0.05). (B) Plaque numbers formed by the ΔccpC strain in L2 fibroblasts is approximately 40% reduced compared to the wildtype F2365 strain (p < 0.05). The C∆ccpC is found to restore the number of plaques to wildtype F2365 level. (C) Plaque sizes formed by the ΔccpC were not significantly different when compared to the wildtype F2365 (p > 0.05). (D) The expression of inlB and inlA genes during the growth of L. monocytogenes strains in BHI broth was significantly downregulated in the ΔccpC strain compared to the wildtype F2365 (p < 0.05). The data represent the mean ± SEM. Data were compared with Student’s t-tests. Asterisks (*) indicate significant differences, p < 0.05. NS, No significant difference.