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. 2024 May 31;15:1403694. doi: 10.3389/fmicb.2024.1403694

Figure 5.

Figure 5

Growth and survival of ΔccpC strain and wildtype F2365 under oxidative stress. (A) The ΔccpC strain exhibited a similar growth curve compared to wildtype F2365 and complement strains when grown in BHI at 37°C without oxidative stress. The ΔccpC strain exhibited a decreased lag phase compared to the wildtype F2365 strain when cultured in BHI broth with 6 mM (B), 8 mM (C), and 10 mM (D) of H2O2. Growth assays were performed in a 48-well plate and were repeated at least six times independently with five replicates. Error bars represent the SEM. The survival of the ΔccpC and wildtype F2365 strains was assessed following exposure to 6, 8, or 10 mM H2O2 for 1 h (E) and 2.5 h (F). L. monocytogenes strains were grown to the mid-log phase, resuspended in BHI, and treated with H2O2, after which CFUs were enumerated by colony counts on BHI agar plates. Statistical analysis was performed using Student t-test.