Figure 4.

SMTNL1 hampers the migration capacity of endometrial epithelial cells. Scratch assay of DiD fluorescent dye-labeled Ishikawa cells transfected with either empty vector (MOCK) or FT-SMTNL1 and maintained in control, progesterone (P4), or gestational diabetes (GDB) environment. After 48 hours of treatment cells were scratched and were grown for an additional 24 h. Surface area of the closed territory was analyzed, and values were normalized and expressed as the ratio of the control MOCK group. Data was plotted as bar charts, where each column represents n=4-9, mean ± SD. Two groups were compared by unpaired two-tailed t-test, and four groups or more were compared by two-way ANOVA, where: p< 0.05 (*), p<0.01 (**) and p<0.0001 (***). (A) Photos of scratch areas were taken at the indicated times. Scale bars: 500 µm (B).