Fig. 6.

Pericyte-specific protein purification and sample evaluation.A, illustration of protein-purification workflow using monomeric avidin beads to capture biotinylated proteins with subsequent elution with first 2 mM biotin solution as a competitive agonist. B, Western blotting with SA-HRP or silver stain were used to evaluate the purification efficiency of supernatant samples after 24 h of hypoxia, originating from monocultures and (C) spheroids of TurboID-pericytes and negative controls before proteomic analysis. E, elution; FT, flow-through; IN, input; W, wash.