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. 2024 May 4;300(6):107343. doi: 10.1016/j.jbc.2024.107343

Figure 4.

Figure 4

The degradation of HMW-PAHs by the resting cells which expressed NarA2B2 and PhtAcAd.A, the degradation percentages of HMW-PAHs by E. coli BL21(DE3) containing pETDuet-narA2-Ter-narB2 and pACYCDuet-phtAcAd at 24 h. B, the heat map of degradation percentages of HMW-PAHs by NarA2B2 variants. PYE: pyrene; FLU: fluoranthene; BaA: benzo[a]anthracene; CHR: chrysene; BbF: benzo[b]fluoranthene; BaP: benzo[a]pyrene. NarA2B2WT: the WT of NarA2B2; NarA2B2A: NarA2B2V236A; NarA2B2B: NarA2B2Y300F; NarA2B2C: NarA2B2W316I; NarA2B2D: NarA2B2L375F; NarA2B2AB: NarA2B2V236A-Y300F; NarA2B2AC: NarA2B2V236A-W316I; NarA2B2AD: NarA2B2V236A-L375F; NarA2B2BC: NarA2B2Y300F-W316I; NarA2B2BD: NarA2B2Y300F-L375F; NarA2B2CD: NarA2B2W316I-L375F; NarA2B2ABC: NarA2B2V236A-Y300F-W316I; NarA2B2ABD: NarA2B2V236A-Y300F-L375F; NarA2B2ACD: NarA2B2V236A-W316I-L375F; NarA2B2BCD: NarA2B2Y300F-W316I-L375F; NarA2B2ABCD: NarA2B2V236A-Y300F-W316I-L375F.