FIG. 6.

RT-PCR and DNA PCR experiments to assess IR2 transcripts and gene presence in NPC tumors, BLs, and BL-derived cell lines. The blots were probed with ³²P-radiolabeled oligonucleotide H-2T, and bands were visualized by autoradiography. (a) RT-PCR products from HEp-2C (track 3) and Daudi (track 4) cells and C15 (track 5), C17 (track 6), C18 (track 7), NPC 1 (track 8), and NPC 2 (track 9) tumors. Other tracks contain PCR products from C15 DNA treated with DNase I (track 2) and untreated (track 10); from C17 (track 11), C18 (track 12), NPC 1 (track 13), NPC 2 (track 14), and Daudi (track 15) DNAs; or from primers only (track 16). (b) RT-PCR products from HEp-2C cells (track 3), Daudi cells (track 4), NPCs 3 to 7 (tracks 5 to 9, respectively), C15 (track 10), or B95-8 cells (track 11). Other tracks contain PCR products from C15 DNA treated with DNase I (track 2) or untreated (track 12) or from primers only (track 13). (c) RT-PCR products from Ramos (track 3), Daudi (track 4), and B95-8 (track 11) cells; CC tumor (track 5); and BL tumors SD, IB, LC, TJ, and GE (tracks 6 to 10, respectively). Other tracks contain PCR products from B95-8 DNA treated with DNase I (track 2) or untreated (track 12) or from primers only (track 13). (d) RT-PCR products from Ramos uninduced (track 3) and induced (track 4) cells, Daudi uninduced (track 5) and induced (track 6) cells, M-ABA uninduced (track 7) and induced (track 8) cells, Raji uninduced (track 9) and induced (track 10) cells, P3HR-1 uninduced (track 11) and induced (track 12) cells, and B95-8 uninduced (track 13) and induced (track 14) cells. Other tracks contain PCR products from B95-8 DNA treated with DNase I (track 2) or untreated (track 15) or from primers only (track 16). In each panel, track 1 contains molecular size markers, as noted for Fig. 5.