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. 2024 Apr 24;18(2):101347. doi: 10.1016/j.jcmgh.2024.04.004

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© 2024 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Telocytes are actively recruited during metaplasia development. (A) Immunofluorescence staining of RFP (red), FOXL1 (white), PDGFRα (green), and DAPI (blue) on L635-treated Foxl1-CreERT2;R26R-tdTomato mouse stomach tissue. The use of RFP antibody to detect the tdTomato expression revealed an extension of labeled Foxl1-expressing telocytes toward the base of the gland, specifically in L635-treated stomach tissue. Scale bar: 100 μm and 50 μm for enlarged. (B) Three-Dimensional whole mount image of Foxl1-CreERT2;R26R-tdTomato mouse stomach tissue after tissue clearing. Endogenous tdTomato (red) and DAPI (cyan) were observed under a confocal microscope. Whereas untreated stomachs mainly exhibited tdTomato-labeled telocytes at the upper part of the gland, L635 treatment showed these telocytes expanded with longer telopodes reaching the gland's mid and base sections. (C) Orthogonal views obtained from different planes (x/y, x/z and y/z) using maximum intensity projection. Endogenous tdTomato (red) signal showed telocytes in the L635-treated stomach extending further to the gland base, contrasting with the TAM-only control.