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. 2024 Apr 24;18(2):101347. doi: 10.1016/j.jcmgh.2024.04.004

Figure 9.

Figure 9

Wnt5a expression colocalizes with PDGFRα-positive telocytes and reflects the telocyte distribution shift in metaplasia. (A) RNAscope fluorescence in situ hybridization of Wnt5a (red) and immunofluorescence staining of PDGFRα (green) and DAPI (blue) on Mist1-Kras 1-month mouse stomach. Wnt5a expression, detected by RNAscope, exhibited colocalization with PDGFRα-positive cells located toward the upper region of the WT control stomach, where telocytes were observed. In the metaplastic Mist1-Kras 1-month stomach, Wnt5a expression extended throughout the gland in PDGFRα-positive cells, encompassing the base of the gland. Scale bar: 100 μm and 50 μm for enlarged. (B) RNAscope fluorescence in situ hybridization of Bmp4 (red) and immunofluorescence staining of PDGFRα (green) and DAPI (blue) on Mist1-Kras 1-month mouse stomach. In the WT control stomach, colocalization of Bmp4 expression with PDGFRα-positive cells was observed throughout the gland. Additionally, Bmp4 expression was noted in PDGFRα-negative cells within the stroma of the WT control stomach. In the metaplastic Mist1-Kras 1-month stomach, Bmp4 expression was enhanced throughout the gland in both PDGFRα-positive cells and within the stromal compartment. Scale bar: 100 μm and 50 μm for enlarged. (C) RNAscope fluorescence in situ hybridization of Bmp7 (red) and immunofluorescence staining of PDGFRα (green) and DAPI (blue) on Mist1-Kras 1-month mouse stomach. Bmp7 expression was not detected in the WT control stomach. In the metaplastic Mist1-Kras 1-month stomach, a general increase in Bmp7 expression was observed in PDGFRα-positive cells and epithelial cells. Scale bar: 100 μm and 50 μm for enlarged.