FIG. 2.

SPR sensograms for wild-type Ad5 fiber knob interacting with sCAR (A) and IgV domain alone (B). Ad5 fiber knob was injected over the sensor surface at multiple concentrations in the range of 10 nM to 5 μM. A 90- to 120-s association phase was followed by a 60-s dissociation phase with HBS buffer flowing over the sensor surface at 10, 20, 30, and 40 μl/min. Representative sensograms of Ad5 fiber knob as analyte at concentrations of 200 nM, 400 nM, and 5 μM are shown.