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SPR analysis of interactions between sCAR and wild-type and mutant Ad5 fiber knobs. sCAR was immobilized on the CM5 chip. The filled bars represent the maximal association response at equilibrium in RU, and the open bars represent the response at a fixed time point (60 s) into the dissociation phase. This permits comparison to be made between wild-type and mutant fiber knob proteins in relation to their association and dissociation kinetics.
