Fig. 6.

A vascularized micro-organ platform showing promise for studying monocyte–endothelial cell (EC) interactions. Unpublished data: a a microfluidics device incorporating arteriole (high pressure) and venule (low pressure) fluid channels drives fluid flow across and b angiogenesis within a flanking cell chamber (red, ECs). c Anastomosis with these channels and physiological tightness of the vessels is shown by perfusion with 70 kDa rhodamine-dextran (green). d Perfusion of 100,000 monocytes per mL demonstrates monocyte adhesion after 18 h of perfusion (green, arrowheads). Closer examination within the dashed area reveals e both adherent (arrowheads) and extravasated (arrows) monocytes. f Both monocyte populations are readily quantified over time (***both adherent and extravasated monocytes increased over time in this preliminary study)