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Colocalization of UL3.5 and αBTIF in transfected cells. Vero cells were transfected with pUL3.5cDNA3 (A), pαBTIFcDNA3 (B), and pUL3.5cDNA3 plus pαBTIFcDNA3 (C). The cells were fixed 40 h after transfection, incubated with both anti-UL3.5 and anti-αBTIF antibodies, stained with Texas Red- and FITC-conjugated secondary antibodies, and examined with a dual-channel confocal microscope.
