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[Preprint]. 2024 Jun 6:rs.3.rs-4467983. [Version 1] doi: 10.21203/rs.3.rs-4467983/v1

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BV-2 cells were treated for 24 hours, and cytokine levels were measured using a Proteome Profiler assay. A and B. Statistical analysis revealed that ONX-0914 treatment abrogated the IFNγ-dependent increase of Ip-10 ([F(3,11)=104.4], p<.001). Post hoc analysis revealed that IFNγ increased Ip-10 levels compared to control (p<.001), ONX-0914 (p<.001), and ONX-0914+IFNγ co-treatment (p<.001). In addition, there was a significant difference of Mig protein levels between treatment groups ([F(3,11)=18.61], p<.001). Post hoc analysis revealed that IFNγ treatment resulted in higher Mig protein levels than all other groups (Ctrl,ontrol p<.001; ONX-0914, p<.001; ONX-0914+IFNγ, p=.003). MCP-1 levels were significantly different between groups ([F(3,8)=5.591], p=.02). IFNγ treatment increased MCP-1 levels compared to control (p=.035) which was reduced by ONX-0914 co-treatment (p=.029). Rantes protein levels were also different between treatment groups ([F(3,12)=24.18], p<.001). Post hoc analysis revealed that Rantes cytokine levels were significantly higher in the IFNγ treatment group compared to all other groups (Control, p<.001; ONX-0914, p<.001; ONX-0914, +IFNγ, p<.0001).. C. BV-2 β5i KO cells were treated with IFNγ for 24 hours, and cytokine levels were measured using a Proteome Profiler assay. Ip-10, Mig, Rantes, and MCP-1 chemokine induction wasere abrogated in BV-2 β5i KO cells exposed to IFNγ, similar to ONX-0914 treatment.