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[Preprint]. 2024 Jun 6:rs.3.rs-4467983. [Version 1] doi: 10.21203/rs.3.rs-4467983/v1

Figure 4. Altered degradation of IκBαin the absence of immunoproteasome activity.

Figure 4

IκBα levels were measured in WT and β5i KO BV-2 cells in the absence and presence of IFNγ. A. Representative Western blot of IκBα in WT BV-2 and β5i KO BV-2 βcells over a 240 minute time course following IFNγ exposure. B. Quantification of the data represented in A. (n=4, *p<0.05, ns=no significance) . There was a significant difference between groups ([F(3,28)=12.75], p<.001). Post hoc analysis revealed that IFNγ treatment significantly reduced IκBα levels compared to control (p<0.05) after 20 minutes. IκBα levels were unchanged in BV-2 β5i KO cells treated with IFNγ. C. Gene expression analysis of cox2C by qRT-PCR (n=5, ***p<0.001, ****p<0.0001, ns=no significance).