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HPV-16 E7 49 to 57 specific CTL responses induced by immunization with rAAV E7CTL-hsp. C57BL/6 mice were i.m. immunized with rAAV E7CTL-hsp. Four weeks after the vaccination, the spleens were collected and analyzed for in vitro CTL assay. (A) TC-1 cells; (B) B16F1 cells; (C) B16F1 cells pulsed with E7 peptide 49 to 57, (D) B16F1 cells pulsed with HPV-16 E5 peptide 6 to 13. The data were the averages of five vaccinated mice. E/T, effector/target. PBS, phosphate-buffered saline.
