FIG. 5.

Proliferation of rAAV E7CTL-hsp-vaccinated lymphocytes in response to E7 peptide 44 to 62. C57BL/6 mice were i.m. immunized with rAAV E7CTL-hsp, rAAV lacZ, or mock. Four weeks after the vaccination, the spleens were collected and analyzed for T-cell proliferation measured by incorporation of [³H]thymidine. The data were the averages of five vaccinated mice of each group. The error bars represent standard errors. Stimulation index was defined as the ratio of mean counts per minute of incorporated [³H]thymidine for cells with antigen (E7 peptide 44 to 62) to mean counts per minute for cells without any added antigen. PBS, phosphate-buffered saline.