Skip to main content
. 2024 Jun 14;19(6):e0304021. doi: 10.1371/journal.pone.0304021

Fig 3. Effects of S. marianum, S. eburneum, and S. marianum commercial seed oils on oxysterols (7-cetocholesterol and 7β-hydroxycholesterol) induced ROS overproduction in THP-1 cells.

Fig 3

THP-1 cells were incubated for 24 h with or without 7KC et 7β-OH (62.5μM) in the presence or absence of Silybum seed oils (SMSO, SESO, and SMCSO) (100 μg/mL) or α-tocopherol (400 μM). The histogram represented the percentage of DHE-positive cells. The results are expressed as mean ±SD of three independent experiments. Different letters indicate significate difference between the groups (p <0.05, Tukey test, multiple comparative analysis). a: comparison to control; b: comparison to DMSO (0.166%); c: comparison to Eth (0.104%); d: comparison to “DMSO (0.166%) +Eth (0.104%)”; e: comparison to α-tocopherol (400 μM); f: comparison to SMSO (100 μg/mL); g: comparison to SESO (100μg/mL); h: comparison to SMSO (100 μg/mL); i: comparison to 7KC (62.5 μM); j: comparison to “7KC + α-tocopherol (400 μM)”; k: comparison to “7KC + SMSO (100 μg/mL)”; l: comparison to “7KC + SESO (100 μg/mL)”; m: comparison to “7KC + SMCSO (100 μg/mL)”; n: comparison to 7β-OH (62.5 μM); o: comparison to “7β-OH + α-tocopherol (400 μM)”; p: comparison to “7β-OH + SMSO (100 μg/mL)”; q: comparison to “7β-OH + SESO (100 μg/mL)”; r: comparison to “7β-OH + SMCSO (100 μg/mL)”.