Extended Data Fig. 5. H3K27ac mediated chromatin reorganization during aging.
(a) Measurement of H3K27ac levels in Rag2−/− young (blue) and old (red) pro-B cells via flow cytometry. The IgG isotype controls are shown in dotted lines. The dot plot on the bottom indicates the median fluorescence intensities (MFI) of H3K27ac levels in young and old pro-B cells. Data are presented as mean ± SEM. Unpaired two-sided t test was used, with the P-value indicated. (b) Age-selective p300 ChIP-Seq binding sites were identified in young and old pro-B cells. The analysis and cutoffs used were consistent with those in Fig. 4a. (c) Overlaps of young selective H3K27ac and p300 peaks.(d) Age-selective Brg1 ChIP-Seq binding sites were identified in young and old pro-B cells. The analysis and cutoffs used were consistent with those in Fig. 4a. (e) H3K27ac HiChIP heatmaps of the Ebf1 locus in young (upper) and old (lower) pro-B cells. The visualization elements are similar to that shown in Fig. 4h.