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. 2024 Jun 12;26(6):991–1002. doi: 10.1038/s41556-024-01424-9

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — (a) Average utilization of D_H gene segments in V_HDJ_H and DJ_H joins in young and old C57BL/6J pro-B cells by VDJ-Seq (Hu et al.⁴⁹) using J_H1 bait (n = 2). Clone names of D_H gene segments are annotated. (b) Total CDR3 number in young and old C57BL/6J pro-B cells (n = 2). (c) CDR3 length distribution of productive V_HDJ_H exons in young and old C57BL/6J pro-B cells by J_H1 bait (n = 2). Bar graph represents the mean of two replicate experiments, with each replicate shown as a dot.

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