FIG. 5.

ICP27 induces accumulation of unspliced α-globin pre-mRNA in transfected cells. HeLa cells were transfected with the ICP4 expression clone (pBB37) in combination with pUC, the control vector pUHD10-3, or the ICP27 expression clone pC27, and total RNA was extracted at 36 h posttransfection. These RNA samples and RNA from KOS-infected cells were treated with RNase H and oligo(dT) and then analyzed for α-globin transcripts by Northern blot hybridization. C, control blood RNA; M, RNA size markers (in kilobases).