Extended Data Fig. 4. Identification of DHX15 as a binding partner of LOC.
a) Schematic representation of RNA pulldown experiment to identify LOC specific interactor proteins. b) Confirmation of Dyskerin (DKC) protein and Terc RNA interaction by western blot upon Terc pull down. c,d) List of filtered LOC candidate interacting proteins. Numbers represent the Exclusive Unique Peptide Count. e) Kaplan–Meier survival curve of glioblastoma patients from DHX15 high-expression (n = 109) or DHX15 low-expression group (n = 112). f) LOC–MS2 or Terc–MS2 vectors were co-transfected with MS2-GFP vector into 293T cells. 48 hours later IP was carried out using GFP antibody and analysed by western blotting using indicated antibodies. Input shows the levels of relevant proteins. Source unprocessed blots are available in source data. p-values: e, log-rank test.