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. 2024 Jun 14;14(6):e1733. doi: 10.1002/ctm2.1733

FIGURE 5.

FIGURE 5

Nicotine, mercury and cadmium inhibit the functions of isolated guinea pig pancreatic epithelial cells. (A) Average traces and summary bar charts showing that forskolin‐stimulated fluid secretion into the closed luminal space was inhibited by administration of 1 µM nicotine (n = 4), 3.38 µg/mL Cd (n = 3) or 23 ng/mL Hg (n = 5) compared to the control. (B) Representative pHi traces and summary bar charts demonstrating that regeneration from alkalosis and acidosis was significantly reduced by 30 min pretreatment with all three components of cigarette smoke. (C–F) Confocal images showing preincubation of isolated pancreatic ductal fragments with Cd (D), Hg (E) or nicotine (F) significantly reduced the apical localisation of cystic fibrosis transmembrane conductance regulator (CFTR). (G) No‐primary antibody control. Scale bar = 50 µm. Green: CFTR; blue: DAPI. An intensity profile of CFTR localisation confirmed that apical distribution was reduced after nicotine, Hg or Cd preincubation.