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. 2024 Apr 25;134(12):e176355. doi: 10.1172/JCI176355

Figure 2. GOLIM4 is a protumorigenic effector of the 3q amplicon.

Figure 2

(A and B) Correlation analyses of 3q-amplified (red) and -diploid (black) lung cancer cell lines. (A) GOLIM4 copy numbers, mRNA levels (left plot), and protein levels (right plot) from the CCLE database. (B) qRT-PCR analysis of GOLIM4 copy numbers (CN) and mRNA levels. (C) WB analysis of GOLIM4 protein levels in cell lines. NL, normal lung epithelial cells. (D) WB confirmation of target gene depletion in shRNA-transfected H1299 cells. GOLIM4 (shGOLIM4) and control (shCTL) shRNAs were used. (E and F) Orthotopic lung tumor sizes and distant metastasis numbers generated by shRNA-transfected H1299 cells (E) or H520 cells (F) and images of H&E-stained lung sections (E). Scale bar: 4 mm. Arrows indicate tumors. (G) Kaplan-Meier survival analysis of orthotopic H520 tumor–bearing mice. (H) WB confirmation of target gene depletion by CRISPR/Cas9-mediated gene editing in H1299 cells. (I) Sizes of flank tumors generated by the cells in H. Parental (H1299). (J) Size of flank tumors generated by the cells in D. (K) Primary tumor sizes and lung metastasis numbers generated by subcutaneously injected parental and GOLIM4-KO 344SQ cells. Data indicate the mean ± SD from a single experiment incorporating biological replicate samples (n = 3, unless otherwise indicated) and are representative of at least 2 independent experiments. ***P < 0.001, by 1-way ANOVA (E and F), log-rank test (G), or 2-tailed Student’s t test (I–K).