TABLE 1.
The end point titers of antibodies in the immunized mice
| Group no. | DNA and/or protein (no. of injections)a | No. of mice | Anti-E2 IgG titer (102) 3 weeks afterb:
|
||
|---|---|---|---|---|---|
| First injection | Second injection | Third injection | |||
| I | pTV2 (3) | 17 | 2 ± 1 | 4 ± 2 | 9 ± 3 |
| II | pTV2 (2), gDE2t (1) | 8 | 2 ± 1 | 4 ± 2 | 277 ± 67 |
| III | pTV2sE2t (2), gD (1) | 8 | 53 ± 9 | 199 ± 33 | 312 ± 45 |
| IV | pTV2sE2t (2), gDE2t (1) | 17 | 61 ± 12 | 245 ± 44 | 7,003 ± 2,234 |
| V | pTV2sE2t (3) | 17 | 57 ± 11 | 212 ± 33 | 673 ± 121 |
| VI | gDE2t (2), pTV2sE2t (1) | 8 | 232 ± 42 | 1,098 ± 212 | 1,520 ± 235 |
| VII | gDE2t (3) | 17 | 267 ± 55 | 1,123 ± 234 | 3,121 ± 458 |
| VIII | pTV2sE2t (1), gDE2t (1) | 8 | 55 ± 11 | 601 ± 173 | |
a
Female BALB/c mice were injected with 100 μg of DNA (i.m.) or 5 μg of protein (s.c.) and then boosted one time (group VIII) or two times (groups I to VII) with an identical dose of DNA or protein at 4-week intervals.
b
Data shown represent geometric mean titers ± standard errors for each group of animals.