Fig. 2.

In vitro binding competition of DARPin-800CW conjugates on HT-29 and COLO-320 cells. Cells were preincubated with PBS (control), unconjugated Ac2, Ec4.1, or Off7 (each at 200 nM), followed by incubation with DARPin-800CW conjugates (100 nM). a.u, arbitrary units; MFI, mean fluorescence intensity; ns, not significant. **P < 0.01, ***P < 0.001. Experiments were performed in triplicate