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. 2024 Apr 29;16(6):1427–1450. doi: 10.1038/s44321-024-00072-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV5 — (A, B) Mean and SEM with single values of the % (C) or MFI (D) of GFP-positive primary blood outgrowth endothelial cells (BOEC) derived from a hemophilia A donor (n = 2 independent experiments) transduced with VSV.G-LV or GP64-LV at the indicated MOI. (C) Mean and SEM with single values of the amount of hFVIII measured in the culture supernatant of primary BOEC derived from a hemophilia A donor (n = 2 independent experiments) transduced with VSV.G-LV or GP64-LV at the indicated MOI, or left untreated. hFVIII concentration reported is obtained from two supernatant collection. (D) Mean and SEM with single values of the VCN of reversed transcribed LV genome of the primary BOEC (shown in (A–C)) transduced with VSV.G-LV or GP64-LV encoding for GFP or hFVIII at the indicated MOI, or left untreated.