TABLE 1.
Immune reconstitution of SCID-hu micea
| Expt and mouse no. | HIV infected | Drug treated | Results at time point 1
|
Results at time point 2
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| % CD4 SP | % CD4-CD8 DP | % CD4+, CXCR4+ | % CD4+, CCR5+ | % CD4 SP | % CD4-CD8 DP | % CD4+, CXCR4+ | % CD4+, CCR5+ | |||
| Expt 1 | ||||||||||
| 175-4 | + | − | 28 | 1.7 | 15 | 6.4 | 16.8 | 3.0 | 0.8 | 0.0 |
| 175-25 | + | − | 14 | 3 | 2.9 | 0.6 | 0.7 | 0.6 | 0.04 | 0.0 |
| 175-26 | + | + | 28 | 3 | 11 | 4.5 | 12.5 | 5.0 | 44 | 4.2 |
| 175-29 | + | + | 32 | 2.0 | 11 | 4.0 | 44 | 29 | 40 | 0.3 |
| 175-31 | + | + | 7.2 | 1.3 | 1.4 | ND | 17 | 31 | 31 | 5.9 |
| 175-32 | + | + | 33 | 3.9 | 13 | ND | 22 | 62 | 56 | 0.8 |
| 175-33 | − | + | 24 | 72 | 51 | ND | 15 | 83 | 58 | 0.2 |
| 175-36 | − | + | 20 | 77 | 58 | ND | 23 | 72 | 52 | 0.3 |
| 175-28 | − | + | 18 | 76 | 40 | 1.9 | 18 | 69 | 49 | 0.6 |
| Expt 2 | ||||||||||
| 179-8 | + | − | 7 | 10 | 2.1 | 3.7 | 8 | 2.4 | ||
| 179-10 | + | − | 9 | 10 | 2.3 | 5.1 | 1.2 | 13 | ||
| 179-1 | + | + | 11 | 0.7 | 6 | 12 | 70 | ND | ||
| 179-2 | + | + | 1.3 | 0.15 | 7 | 8 | 33 | ND | ||
| 179-4 | + | + | 1.3 | 0.3 | 15 | 7.7 | 56 | 3.1 | ||
| 179-6 | + | + | 8.1 | 0.6 | 7 | 18 | 29 | ND | ||
| 179-25 | + | + | 20 | 57 | 8.2 | 11 | 57 | 4.8 | ||
| 179-27 | + | + | 5.3 | 0.8 | 10 | 6.7 | 66 | ND | ||
| 179-28 | + | + | 2.1 | 2.9 | 13 | 11 | 48 | 7.9 | ||
| 179-30 | + | + | 12 | 1.3 | 6.4 | 17 | 29 | 7.5 | ||
| 179-9 | − | + | 2.9 | 84 | 0.04 | 32 | 46 | ND | ||
| 179-7 | − | + | 5 | 79 | 0.1 | 7.8 | 81 | 0.9 | ||
| 179-12 | − | − | 4.2 | 79 | 0.04 | 7.7 | 67 | 0.5 | ||
Results from two separate experiments, donor series numbers 175 and 179, are shown. Some SCID-hu mice were infected with HIV-1NL4-3 (+), and biopsies were taken at 8 weeks (time point 1) and 11 weeks (time point 2, experiment 1) or 12 weeks (time point 2, experiment 2) postinfection and analyzed by flow cytometry. Mock-infected mice (−) from the same donor series were analyzed in parallel. Immediately following the biopsies 8 weeks postinfection, indicated mice were administered a daily combination of AZT, ddI, and indinavir for the remainder of the experiment. Cells at each time point were stained with antibodies specific for CCR5 (FITC), CXCR4 (PE), CD4 (Red613), and CD8 (APC). Percentages of CD4 single positive (SP) and CD4-CD8 DP cells are indicated for each time point, as are the percentages of CXCR4- and CCR5-expressing cells.