Table 2.
Effect of punicalagin on viral RNA copy number
| Gene | Sample | Ct | Viral genes copy number (log10) | |
|---|---|---|---|---|
| 15 | 7.98±0.19 | 5.18±0.03 | ||
| Virus Control | 8.65±0.50 | 5.09±0.03 | ||
| HA | punicalagin Concentration (µg/ml) | 30 | 9.98±0.27 | 4.71±0.30 |
| 15 | 8.12±0.22 | 5.04±0.06 | ||
| Virus Control | 8.67±2.14 | 5.06±0.36 | ||
| NS1 | punicalagin Concentration (µg/ml) | 30 | 9.92±1.82 | 4.85±0.30 |
Cells were infected with influenza virus and treated with 30 and 15 µg/ml of punicalagin. After 8 hr of incubation, cell culture supernatants were removed and total intracellular RNA was extracted and the Log10 copy numbers of viral RNA quantity in the samples, expressed as log10 TCID50/ml equivalents, is derived by plotting the Ct of a sample on the standard curve, there is no significant difference between the treated and untreated samples (p>0.05, Kruskal-Wallis test).