Figure 3.

Hypomethylated regions in spermatids/sperm are enriched for specific TF binding sites

(A) Depicted are the enriched sequences of known motifs identified by HOMER. HOMER analysis was run for all DMR comparisons and significant results are displayed (p value <0.01, false discovery rate (FDR) < 0.05). Undiff, undifferentiated spermatogonia; Diff, differentiating spermatogonia; 4C, primary spermatocytes; 1C, spermatids/sperm.

(B) Dot plots show the average single-cell expression¹⁶ of the top three transcription factors (TFs) with enriched motifs among the DMRs identified with HOMER. SPC, spermatocytes; SPD, spermatids.