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. 2024 May 31;12:1385712. doi: 10.3389/fcell.2024.1385712

FIGURE 4.

FIGURE 4

HUVEC capillary-like formation assay. (A) Representative images of HUVECs on BME treated with conditioned medium (CM) or (B) exosomes (EXOs). (C–H)- Graphs represent a quantitative analysis of the (C) number of nodes, (D) number of branches, (E) number of meshes, (F) master segment length, (G) branch length, and (H) tube length. Untreated serum-free DMEM (NT); Untreated DMEM with serum (positive ctrl); Serum-free DMEM conditioned by hAMSCs (CM with EXOs); Serum-free DMEM conditioned by hAMSCs depleted of EXOs (CM w/o EXOs); Serum-free DMEM conditioned by hypoxic hAMSCs (HYP CM with EXOs); Serum-free DMEM conditioned by hypoxic hAMSCs depleted of EXOs (HYP CM w/o EXOs); Serum-free DMEM conditioned by IFN-γ-treated hAMSCs (IFN-γ CM with EXOs); Serum-free DMEM conditioned by IFN-γ-treated hAMSCs depleted of EXOs (IFN-γ CM w/o EXOs); 30 μg/mL EXOs secreted by hAMSCs (EXOs 30 μg/mL); 60 μg/mL EXOs secreted by hAMSCs (EXOs 60 μg/mL); 30 μg/mL EXOs secreted by hypoxic hAMSCs (HYP EXOs 30 μg/mL); 60 μg/mL EXOs secreted by hypoxic hAMSCs (HYP EXOs 60 μg/mL); 30 μg/mL EXOs secreted by IFN-γ-treated hAMSCs (IFN-γ EXOs 30 μg/mL); 60 μg/mL EXOs secreted by IFN-γ-treated hAMSCs (IFN-γ EXOs 60 μg/mL). Data are presented as the means ± SD of triplicate in two independent experiments. ∗p < 0.05 vs. NT.