FIGURE 5.

HUVEC migration assay. (A) Real-time migration monitoring of HUVECs with the xCELLigence system. (B) Slopes of migration curves. Untreated serum-free DMEM (NT); Untreated DMEM with serum (positive ctrl); Serum-free DMEM conditioned by hAMSCs (CM with EXOs); Serum-free DMEM conditioned by hAMSCs depleted of EXOs (CM w/o EXOs); Serum-free DMEM conditioned by hypoxic hAMSCs (HYP CM with EXOs); Serum-free DMEM conditioned by hypoxic hAMSCs depleted of EXOs (HYP CM w/o EXOs); Serum-free DMEM conditioned by IFN-γ-treated hAMSCs (IFN-γ CM with EXOs); Serum-free DMEM conditioned by IFN-γ-treated hAMSCs depleted of EXOs (IFN-γ CM w/o EXOs); 30 μg/mL EXOs secreted by hAMSCs (EXOs 30 μg/mL); 60 μg/mL EXOs secreted by hAMSCs (EXOs 60 μg/mL); 30 μg/mL EXOs secreted by hypoxic hAMSCs (HYP EXOs 30 μg/mL); 60 μg/mL EXOs secreted by hypoxic hAMSCs (HYP EXOs 60 μg/mL); 30 μg/mL EXOs secreted by IFN-γ-treated hAMSCs (IFN-γ EXOs 30 μg/mL); 60 μg/mL EXOs secreted by IFN-γ-treated hAMSCs (IFN-γ EXOs 60 μg/mL). Data are presented as the means ± SD of quadruplicate in two independent experiments. ∗p < 0.05 vs. NT.