FIGURE 7.

Secretion clusters (z-scores) of both up- and downregulated proteins in peripheral blood. Quantification of soluble factors secreted by peripheral blood cells stimulated or unstimulated with LPS in the presence or absence of each treatment after (A) four and (B) 24 h of culture. (C) Dynamic variations of factors between 4 and 24 h. (D) Correlation matrix of the factors. The degree of correlation between the two factors is shown through color intensity and the diameter of the circles. Significance was analyzed using a Spearman rank test, and the level of significance was set at p < 0.05. Whole blood without treatments (ctrl); Whole blood with serum-free DMEM conditioned by hAMSCs (CM with EXOs); Whole blood with serum-free DMEM conditioned by hAMSCs depleted of EXOs (CM w/o EXOs); Whole blood with serum-free DMEM conditioned by hypoxic hAMSCs (HYP CM with EXOs); Whole blood with serum-free DMEM conditioned by hypoxic hAMSCs depleted of EXOs (HYP CM w/o EXOs); Whole blood with serum-free DMEM conditioned by IFN-γ-treated hAMSCs (IFN-γ CM with EXOs); Whole blood with serum-free DMEM conditioned by IFN-γ-treated hAMSCs depleted of EXOs (IFN-γ CM w/o EXOs); Whole blood with 30 μg/mL EXOs secreted by hAMSCs (EXOs 30 μg/mL); Whole blood with 60 μg/mL EXOs secreted by hAMSCs (EXOs 60 μg/mL); Whole blood with 30 μg/mL EXOs secreted by hypoxic hAMSCs (HYP EXOs 30 μg/mL); Whole blood with 60 μg/mL EXOs secreted by hypoxic hAMSCs (HYP EXOs 60 μg/mL); Whole blood with 30 μg/mL EXOs secreted by IFN-γ-treated hAMSCs (IFN-γ EXOs 30 μg/mL); Whole blood with 60 μg/mL EXOs secreted by IFN-γ-treated hAMSCs (IFN-γ EXOs 60 μg/mL).