Schematic representation of the full-length DNA of pPRKflc2, pPRKflc23 and pPRKflc22. pPRKflc2 is the wild-type full-length DNA copy of CSFV strain C. The amino acid sequence numbering is for the open reading frame of the CSFV strain C (19). The starts of the Erns and E1 proteins are based on N-terminal sequencing (24), but the carboxy termini of the capsid and Erns proteins have not yet been determined. Npro, autoprotease; C, capsid protein; Erns, E1 and E2 envelope proteins; p7, nonstructural protein p7; 5′, 5′ noncoding region; 3′, 3′ noncoding region.