FIG. 5.

Quantitation of apoptosis in HeLa cells transiently expressing IκBα-ΔN. Cells (1 × 10⁶) were either transfected with 5 μg of pHook-2/IκBα-ΔN or 5 μg of pHook-2/lacZ. After 24 h, transfected cells were isolated using Capture-Tec magnetic beads and plated in 24-well plates. (A) Transfected cells (2 × 10⁴) were either mock infected or infected with T3D at an MOI of 100 PFU per cell. After incubation at 37°C for 24 h, the cells were stained with acridine orange. (B) Transfected cells (2 × 10⁴) were either not treated or treated with 20 ng of TNF-α per ml. After incubation at 37°C for 24 h, the cells were stained with acridine orange. The results of the experiments in both panels are expressed as the mean percentage of cells undergoing apoptosis in three independent experiments. Error bars indicate standard error of the mean.