FIG. 7.

(A) NF-κB gel shift activity in reovirus-infected p65+/+ and p65−/− immortalized fibroblast cells. Cells (5 × 10⁶) were either mock infected or infected with T3D at an MOI of 100 PFU per cell. After incubation at 37°C for 8 h, nuclear extracts were prepared and incubated with a ³²P-labeled DNA probe consisting of the NF-κB consensus sequence. Incubation mixtures were resolved by acrylamide gel electrophoresis, dried, and exposed to film. NF-κB-containing complexes are indicated. (B) Quantitation of apoptosis in reovirus-infected p65+/+ and p65−/− cells. Cells (2.5 × 10⁴) were either mock infected or infected with T3D at an MOI of 100 PFU per cell. After incubation at 37°C for 48 h, the cells were stained with acridine orange. (C) Quantitation of apoptosis in TNF-α-treated p65+/+ and p65−/− cells. Cells (2.5 × 10⁴) were either untreated or treated with 20 ng of TNF-α per ml. After incubation at 37°C for 24 h, the cells were stained with acridine orange. The results of the experiments in panels B and C are expressed as the mean percentage of cells undergoing apoptosis in three independent experiments. Error bars indicate standard error of the mean.