Table 1.
Sphingolipids and sphingoid bases in Caco-2/TC7 cells and basolateral medium of cells incubated with deuterated sphingosine-enriched lipid micelles
| Sphingolipids | MM + SPH-d9 |
MM + SPH-d9 + C23:0 |
|---|---|---|
| pmol/well | ||
| Cells | ||
| SPH-d9 | 53.2 ± 2.7 | 58.2 ± 2.6 |
| S1P-d9 | 7.3 ± 0.3 | 5.4 ± 0.9 |
| d18:1 Cer-d9 | 2,850 ± 218 | 2,880 ± 125 |
| d18:1 SM-d9 | 1,039 ± 43 | 1,185 ± 108 |
| Basolateral medium | ||
| SPH-d9 | ND | ND |
| S1P-d9 | ND | ND |
| d18:1 Cer-d9 | 1,159 ± 62 | 1,228 ± 15 |
| d18:1 SM-d9 | ND | ND |
Deuterated SPH (SPH-d9), deuterated S1P (S1P-d9), deuterated Cer (d18:1 Cer-d9), and deuterated SM (d18:1 SM-d9) in cells and basolateral medium of Caco-2/TC7 cells incubated for 16 h with MM enriched with SPH-d9 (MM + SPH-d9) or with SPH-d9 and C23:0 (MM + SPH-d9 + C23:0). MM preparation containing 75 nmol SPH-d9 was added in 1.5 ml apical medium. Data expressed as pmol/well, represent the mean ± SEM of one experiment realized in quadruplicate. No significant difference was observed between sphingolipids in (MM + SPH-d9) and (MM + SPH-d9 + C23:0) groups.
MM, mixed micelles; ND, not detectable; SPH, sphingosine; SPH-d9, deuterated sphingosine.