TABLE 3.
Evolution of the DEN-2 PDK-53 virus vaccine during passage of the parental 16681 virus in PDK cells
| Virus | Genome nt position,a translated polypeptide position,b encoded amino acidsb
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 57 | 524, prM-29, D–V | 2055, E-373, F–F | 2579, NS1-53, G–D | 4018, NS2A-181, L–F | 5270, NS3-250, E–V | 5547, NS3-342, R–R | 6599, NS4A-75, G–A | 8571, NS5-334, V–V | |
| 16681 | C | A | C | G | C | A | T | G | C |
| PDK-5 | C | A | C/Tc | G | T/C | A | T/C | G | C/T |
| PDK-10 | C | A | T/C | G | T | A | C/T | G | T/C |
| PDK-14 | C | A | T | G | T | A | C | G | T/C |
| PDK-35 | T | T/A | T | A/G | T | A | C | C/G | T |
| PDK-45 | T | T | T | A | T | A/T | C | C | T |
| PDK-53 | T | T | T | A | T | A/T | C | C | T |
a
Of the nine nucleotide sequence differences between 16681 and PDK-53 viruses (32). Nucleotide position 57 lies within the 5′NC of the viral genome. A, C, G, and T (cDNA sense) nucleotides are indicated.
b
The virus-specific amino acid residues (16681–PDK-53) are shown for each amino acid position.
c
Two genetic populations were identified for this locus in the virus. The order of the two nucleotides reflects relative peak heights of the nucleotide signals in sequence chromatograms.