FIG. 1.

Mutations in E1 cytoplasmic domain. (A) Amino acid sequence of the E1 cytoplasmic domain and the substitutions produced by mutagenesis. Numbering is based on the sequence published by Clarke et al. (5). (B) Vero cells were transfected with RNAs using Lipofectin and overlaid with growth medium. After 5 days of transfection, the medium was removed for virus titration. Mean titers of two independent plaque assays are shown. (C) Plaque sizes of some of the mutants shown in Fig. 2.