FIG. 6.

Electron microscopic analysis of intracellular virus budding. Transfected BHK cells were treated with 2% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.2), immediately scraped from the plate, and kept at 4°C for 15 min. After being pelleted, the cells were postfixed, dehydrated, and infiltrated with Epon. Sections were cut, stained, and observed by electron microscopy. (A) 24S/WT; (B) 24S/Y472A; (C) 24S/Y473S. Images were scanned with a UMAX Astra 1220U scanner with Adobe Photoshop 5.0 software.