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. 2024 Jun 15;15:5115. doi: 10.1038/s41467-024-49298-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A NF2 KO Schwann cells have reduced AKR1C1, 2 and 3 mRNA levels compared to isogenic NF2-WT controls, assayed by Q-RT-PCR. Bars = mean ± SD. Dots = biological replicates. Significance by one-way ANOVA and Dunnett’s multiple comparisons test. n = 4 biological replicates B NF2 KO Schwann cells have reduced AKR1C3 and increased ACSL3 protein levels compare to isogenic NF2-WT controls. C NF2-KO Schwann cells have reduced ME1 mRNA levels, assayed by Q-RT-PCR, compared to NF2 WT isogenic controls. Bars = mean ± SD. n = 3 biological replicates, Significance by one-way ANOVA and Dunnett’s multiple comparisons test. D NF2-KO Schwann cells have reduced levels of ME1 protein compared to NF2-WT isogenic controls. E NF2-KO Schwann cells have reduced ME1 activity compared to NF2 WT isogenic controls. Bars = mean ± SD. n = 7 biological replicates. Significance by two-way ANOVA and Tukey’s multiple comparisons test. F NF2-KO Schwann cells have reduced G6PD activity compared to NF2 WT isogenic control cells. Bars = mean ± SD. Significance by one-way ANOVA and Dunnett’s multiple comparisons test. n = 5 biological replicates. G NF2-KO Schwann cells have a reduced NADPH/NADP⁺ ratio compared to isogenic controls. Dots = biological replicates. Bars = mean ± SD. Significance by one-way ANOVA and Dunnett’s multiple comparisons test. n = 8 biological replicates. H ME1 mRNA levels are significantly reduced in NF2 mutant and cystic vestibular schwannomas from patients compared to control vestibular nerve. Data are re-analyzed from³³. Dots = individual patients. Bars = mean ± SD Significance by Brown-Forsythe and Welch ANOVA and Dunnetts’s multiple comparisons test. I AKR1C1, 2 and 3 mRNA levels are consistently reduced in different types of vestibular schwannomas (VS: sporadic, cystic and NF2) compared to control vestibular nerve. Data are re-analyzed from³³. Dots = individual patients. Bars = mean ± SD. Significance by Brown-Forsythe and Welch ANOVA and Dunnetts’s multiple comparisons test. Source data are provided as a Source Data file.